Plasmolysis " Wet Lab"
Exploratory Activity: Osmosis in Elodea Cells or Onion Cells
Introduction: One of the functions of the cell membrane is to control the flow of materials into and out of the cell. In this investigation, you will observe the effects of placing plant cells in solutions of various concentrations.
Materials: Elodea leaves or red onion, microscope slides, cover slips, microscope, distilled water or tap water, 2% salt solution, paper towel.
Methods: Prepare a wet mount of an Elodea leaf or red onion skin with tap water. Observe the leaf at 40X and record your observations. Increase the magnification to 100X, observe, and record your observations.
Remove the slide from the stage of the microscope. Place 2 drops of the 5% salt solution on the slide at the edge of the cover slip. Tear off a small piece of paper towel and place the torn edge on the slide at the edge of the cover slip that is opposite the side where the salt solution was placed. The piece of towel should begin to soak up water, drawing the salt solution under the cover slip as it does so.
Introduction: One of the functions of the cell membrane is to control the flow of materials into and out of the cell. In this investigation, you will observe the effects of placing plant cells in solutions of various concentrations.
Materials: Elodea leaves or red onion, microscope slides, cover slips, microscope, distilled water or tap water, 2% salt solution, paper towel.
Methods: Prepare a wet mount of an Elodea leaf or red onion skin with tap water. Observe the leaf at 40X and record your observations. Increase the magnification to 100X, observe, and record your observations.
Remove the slide from the stage of the microscope. Place 2 drops of the 5% salt solution on the slide at the edge of the cover slip. Tear off a small piece of paper towel and place the torn edge on the slide at the edge of the cover slip that is opposite the side where the salt solution was placed. The piece of towel should begin to soak up water, drawing the salt solution under the cover slip as it does so.
Methods (continued): Return the slide to the microscope stage and repeat the observations of the cells at 40X and 100X. Record your observations. You may find it even more dramatic to look at 400x. Repeat the above procedure with the other plant sample (Elodea or onion). Record your observations. Remove the slide from the stage, clean it and the cover slip, and put it away. Put the microscope on low power and put it away.
Observations:
Prepare sketches of a group of Elodea cells or Onion cells under each set of conditions. Label the sketches to note the cell structures that you can identify. Be sure to note any changes in the color, size, and shape of the cells. Make your sketches as accurate as possible.
This can be made much more quantitative by measuring the distance from the tonoplast (the membrane around the vacuole) to the plasma membrane using an ocular micrometer. The images and directions for doing this are below.
Observations:
Prepare sketches of a group of Elodea cells or Onion cells under each set of conditions. Label the sketches to note the cell structures that you can identify. Be sure to note any changes in the color, size, and shape of the cells. Make your sketches as accurate as possible.
This can be made much more quantitative by measuring the distance from the tonoplast (the membrane around the vacuole) to the plasma membrane using an ocular micrometer. The images and directions for doing this are below.
what an ocular micrometer looks like - will be inserted into one of the eyepieces of your objective microscrope
|
what you see through the ocular micrometer (lens)
|
a stage micrometer (slide) and what it looks like under the microscope
|
Conclusions: Answer the following questions.
- What is the shape of the typical Elodea cell or Onion cell?
- What are the small green blobs found inside the cells? What is their function?
- What happens to the cells as the salt water flows under the cover slip?
- What happens to the cells if/when the salt water is flushed out with distilled water?
- Elodea normally lives in fresh water. What changes would you observe in the cells of an Elodea plant that was suddenly moved from fresh water to salt water? Why?
- When cutting onions, your eyes may cry. What is in the onion that makes you cry? Would salt do anything to that substance that makes you cry?
Plasmolysis Dry Lab
experimental design
1. suggest a general research question.
2. suggest a specific research question.
3. identify the independent variable;
is the range sufficient?
4. identify the dependent variable; are the sufficient replicates?
5. what other variables could influence results? how could they be controlled?
2. suggest a specific research question.
3. identify the independent variable;
is the range sufficient?
4. identify the dependent variable; are the sufficient replicates?
5. what other variables could influence results? how could they be controlled?
data analysis
1. where do the values for the x axis come from?
2. where do the values for the y axis come from?
a. what does a positive change in mass mean?
b. what does a negative change in mass mean?
3. What can be learned from finding the x-intercept?
4. how should error be represented on each axis?
2. where do the values for the y axis come from?
a. what does a positive change in mass mean?
b. what does a negative change in mass mean?
3. What can be learned from finding the x-intercept?
4. how should error be represented on each axis?