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          • Types of Errors - more notes.
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        • Natural Selection Web Activities
        • Paper Worm Simulation - Data Collection and Processing
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        • Hominid Evolution - Skull Lab
      • Phylogeny >
        • Constructing Cladograms
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        • Comparative look at animal developmental switches
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Before you get started...try this and learn about the equipment and process

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WET LAB VERSION:

FOLLOW THE INSTRUCTIONS GIVEN IN CLASS FOR THE PARTICULAR DESIGN OF ELECTROPHORESIS  APPARATUS (SEE PHOTO ABOVE, LEFT), THE PARTICULAR MICROPIPETOR MODEL AND PRACTICE DYES AND THE PARTICULAR SAMPLE DNAS (BACTERIOPHAGE LAMBDA DNA, CUT WITH VARIOUS RESTRICTION ENZYMES) THAT ARE AVAILABLE.  A GOOD BASIC GEL RECIPE IS A 1% AGAROSE IN A 1X TBE (TRIS-BORATE-EDTA) BUFFER.  FOR THE CONCENTRATIONS OF PRECUT DNA AVAILABLE COMMERCIALLY, A GOOD LOADING VOLUME IS 5-10 MICROLITERS.

      ONCE YOUR GEL WITH DNA SAMPLES HAS RUN AND BEEN STAINED WITH THE METHYLENE BLUE,  MAKE A SKETCH (OR A PHOTO IF A CAMERA IS AVAILABLE) AND COMPARE THE PATTERN OF RESTRICTION FRAGMENTS (RFLP'S - SEE PHOTO ABOVE, RIGHT) THAT YOU SEE WITH WHAT IS EXPECTED FOR THE GIVEN RESTRICTION DIGEST OF LAMBDA PHAGE DNA.  

ASSIGNMENT:  TURN IN YOUR PHOTO OR SKETCH (USE ANOTHER GROUP'S IF YOUR BANDS WERE TOO FAINT TO SEE) AND ANNOTATE THE IMAGE WITH THE SIZES THAT YOU DEDUCED FOR EACH BAND, BASED ON COMPARISON TO A DIGEST PATTERN THAT YOU FOUND IN A WEB SEARCH FOR THE SAME RESTRICTION ENZYME.
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Eco RI in left two lanes, Pst I on right two lanes, 1 % agarose/TBE gel, 5-10 microliters DNA
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lambda/EcoRI
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lambda/PstI
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lambda/HindIII

Dry lab version: 

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click to link to demonstration exercise
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  • Home
  • SL Bio/Bio 2
    • SL/HL test review
    • SL Bio Blog/Review Discussion Site
    • Name that Sound
    • SL Bio - Topics >
      • Cells >
        • Surface Area/Volume Lab >
          • Types of Errors - more notes.
        • Processing Microscope Data
        • Soap Bubble Membranes
        • Plasmolysis Lab
        • Surface Area/Volume LabHL16 >
          • Types of Errors - more notes.
        • Cell Cycle Timing Lab
      • Biochemistry >
        • Enzyme Lab
        • Photosynthesis Lab
      • Genetics
      • Evolution
      • Ecology SL/Bio2
      • Physiology >
        • Sheep Heart dissection
        • Sheep Brain Dissection
      • SL Option A Neurobiology
  • SL Bio/Bio 2 (pre-2015 exams)
    • SL Bio - Topics >
      • Evolution >
        • Natural Selection Web Activities
        • Paper Worm Simulation - Data Collection and Processing
        • Coacervate Lab
        • Hominid Evolution - Skull Lab
      • Classification
      • Biochemistry >
        • Surface Tension Lab
        • Molecules of Life - Modeling Activity
        • Jmol molecule visualizations
        • Playdough and Toobers!
        • Enzyme Lab
      • SL Genetics >
        • Sockosomes/Chromosocks
      • Biotechnology >
        • DNA gel
        • Bacterial transformation lab
      • Physiology >
        • Digestion
        • Circulation/Gas Exchange >
          • Sheep Heart dissection
        • Nervous system
        • Nutrition - Option A
        • Defense Against Disease
        • Reproduction
      • Ecology >
        • Ecology DBQ practice
        • Population Growth Simulation
        • Emerging Disease Webquest
  • HL BIo Year 1 (pre-2015 exams)
    • Year 1 - Topics >
      • Cells >
        • Processing Microscope Data
        • Surface Area/Volume Lab >
          • Types of Errors - more notes.
        • Plasmolysis Lab
        • Cell Cycle Timing Lab
      • Biochemistry >
        • Surface Tension Lab
        • Molecules of Life - Modeling Activity
        • Jmol molecule visualizations
        • Playdough and Toobers!
        • Race for the Double Helix movie
        • Extraction
      • Metabolism >
        • Enzyme Lab
        • Photosynthesis/Respiration Lab
      • Genetics >
        • Sockosomes/Chromosocks
        • Corn genetics
        • Make a Face
      • Biotechnology >
        • DNA gel
        • Bacterial transformation lab
      • Ecology >
        • Ecology DBQ practice
        • Emerging Disease Webquest
        • Symbiosis in Termites
        • Population Growth Simulation
    • MSON Bioethics >
      • Ethics in Scientific Research
      • Bioethics Films
  • HL Bio Year 1
    • Year One Blog/Review Discussion SIte
    • Year 1 - Topics >
      • EvolutionHL2016
      • CellsHL2016 >
        • Cells: Class Flip Videos
        • Processing Microscope Data HL16
  • HL Bio Year 2
    • SL/HL test review
    • Year Two Blog/Review Discussion Site
    • Ecology Summer Work
    • Year 2 - Topics >
      • Plants
      • Biotechnology_ >
        • DNA gel_
        • Bacterial transformation lab_
        • Microarray
        • Natural Cloning
      • Physiology >
        • Sheep Heart dissection
        • Kidney Dissection
        • Sheep Brain Dissection
        • Chicken Wing Dissection
        • Sea Urchin Embryology
  • HL Bio Year 2 (pre-2015 exam)
    • Year 2 - Topics >
      • Evolution >
        • Natural Selection Web Activities
        • Paper Worm Simulation - Data Collection and Processing
        • Coacervate Lab
        • Hominid Evolution - Skull Lab
      • Phylogeny >
        • Constructing Cladograms
      • Plants >
        • Comparative look at animal developmental switches
        • Plant Microscope exercise
        • Plant Hormone Lab
        • Transpiration Lab
        • Plants and Micrographic Art
        • Plants and Human Art
      • Neurobiology >
        • Preview Topics
        • Neuron Action Potential Simulation
        • Connectome
        • Sheep Brain Dissection
        • Cow Eye Dissection
        • Invertebrate Behavior Lab
        • Altered Reality
        • Human Behavior Lab
      • Physiology >
        • Digestion Demos
        • Sheep Heart dissection
        • Blood typing
        • Kidney Dissection
        • AIDS Global Awareness
        • Virtual ELISA
        • Film - Politics, People and Science of AIDS
        • Chicken Wing Dissection
        • Sea Urchin Embryology
      • Review Project >
        • Review assignments, written by HL students
        • Web 2.0 Tools
  • IB Connections
    • TOK Connections >
      • Natural Sciences - TOK
    • MYP reference site
    • Extended Essay in Biology
    • Group 4 Project
    • Internal Assessment - 1st and 2nd year >
      • Advice on Internal Assessment
      • Uncertainty in calculations
      • t test/TI 83/excel
      • tips on mac excel graphs
      • Notes from IB worldwide report
      • Lab photo album
    • Advice for IB Papers >
      • Advice on IB Examinations
    • IB Learner Profile - How does HL Bio fit in?
  • Resources
    • for our SL friends
    • BioNinja
    • iBiology
    • Click4biology
    • Biozone Weblinks
    • IB Biology video review
    • Smullen Powerpoint
    • Wapole text
    • IB Guides
    • Recommended biology tutorials (besides IB)
    • Practice Tests/Exam Dates/Review Materials >
      • Review assignments, written by HL students
      • Knowledge audits
      • Biology Narratives - Review Activity
      • Organizational review assignment
      • Year 1 Final Review
      • Quizlets and Other Shared Reviews
  • Clubs and Committees
    • Science Olympiad >
      • 2012 Science Olympiad Team in action
      • Photos from the 2013 Olympiad
    • Bioethics Bowl >
      • Villanova Bioethics Competition >
        • Team Pictures - 2012
      • Peace and Ecology Committee
  • Biotechnology