E. COLI TRANSFORMATION USING PGREEN PLASMID - A BIT OF BACKGROUND. SEE ALSO THE INTRO MATERIAL IN THE LAB PROTOCOL DOCUMENT BELOW.
Protein structure - note the alpha helices and the beta sheet/barrel - and the subgroup that is photoreactive - the chromophore.
Bald mice (GFP is not expressed in the fur) - the glowing individuals were transformed as embyos with the gene for GFP. Link to a slide show of glowing organisms...
|
Purified GFP in a test tube - actual protein, not the gene!
|
LInk to interactive demonstration of transformation process (just kidding - here is the link with English annotations - but - can you deduce the translations from the Polish version?)
LAB PROTOCOL - TRANSFORM INTO A PROCEDURAL FLOW CHART BEFORE BEGINNING THE LAB WORK. INCLUDE ONLY THE BASIC INFORMATION FOR EACH STEP, SUCH AS TYPE OF TUBE, VOLUME, CHEMICALS USED, TIME, TEMPERATURE, ETC. SHOULD FIT ON THE PAPER PROVIDED (MAYBE SPILLING OVER ONTO THE BACK IF YOUR HANDWRITING IS LARGE).
ASSIGNMENT: ONCE YOU HAVE YOUR FINAL DATA (PETRI PLATES AFTER DAY 2), COMPLETE THE QUESTIONS ON PAGE 6 OF THE DOCUMENT ABOVE AND THE CALCULATION OF THE TRANSFORMATION EFFICIENCY (PAGE 7). ALSO MAKE ANY AMENDMENTS/ADDITIONS TO YOUR PROCEDURE FLOW CHART AND TURN IT IN.
Alternate method used for plant tissue - Gene Gun
|
|
|
Or mammalian cells - Adenovirus
